The first issue of the Jahrbücher des Nassauischen Vereins für Naturkunde from 1844 contained data from three weather stations in Nassau as well as explanations of the water levels of the largest rivers in the state; it also reported on the flowering and ripening times of wild and cultivated plants and the migration times of migratory birds. After just a few years, the range of topics became broader and the essays more sophisticated, as more and more authors were qualified scientists. The size of the volumes also increased steadily. Particularly in the early days of the association, entire epochs were dominated by scientific heavyweights. For example, Leopold Fuckel described his observations on flowering plants and fungi from 1856-77, Adolph Schenk wrote standard works on bees from 1850-68 and Arnold Pagenstecher devoted himself intensively to butterflies from 1877-1912. In the field of geology/paleontology, the brothers Fridolin von Sandberger and Guido Sandberger wrote numerous articles from 1846-95. From 1846, members of the Fresenius family, in particular Hermann and Carl Remigius Fresenius, published their ongoing observations of Wiesbaden's mineral springs and hydrochemical data. In the years 1931-79 and 1929-69, Fritz Kutscher and Franz Michels, who became known for their numerous essays on geology and hydrogeology, should be mentioned as examples.

The thematic focus of the articles in the yearbooks was on botany and zoology, meteorological phenomena and the Wiesbaden climate, geology, palaeontology, mineralogy, mining and groundwater or mineral and thermal waters. The special volumes "Geology and hydrothermal mineralization" was published in 1998, "Streifzüge durch die Natur von Wiesbaden und Umgebung" in 2004 (2nd edition 2012) and "Zwischen Mittelrhein und Taunus - Naturschätze in Lorch am Rhein" in 2016. The anniversary volume celebrating the 100th anniversary of the association featured a portrait of Baron Anton von Breidbach-Bürresheim, paying tribute to his efforts to found the association and museum and showing the mammoth seal designed by Wiesbaden artist Willy Mulot in 1928 as the association's emblem for the first time. Walter Czysz documented the history of the association and the collection, which has been called the Natural History Collections at the Museum Wiesbaden again since 2012, in detail in the 175th anniversary volume in 2004.

Currently (as of 2015), the yearbooks of the Nassauischer Verein für Naturkunde are requested by around 150 scientific institutions around the world.