The Diakoniegemeinschaft Paulinenstift (formerly Paulinenstift) is a Protestant church foundation under public law. It was founded in 1857 as a hostel for maidservants and for the care of the poor and sick in Schwalbacher Straße. It is named after Pauline Friederike, Duchess of Nassau, who campaigned for the establishment of the institution but died before it was founded.

In 1858, the foundation acquired a property in what later became Stiftstrasse and expanded its activities to include the training of maids, child education and inpatient nursing care. In 1884, the foundation opened its own hospital. In 1896, the foundation moved to its current location in Schiersteiner Straße, where larger facilities and the hospital were built. The motherhouse with the chapel and the educational building (1893-96), the rectory (1901/02) and the children's and infants' home (1909/10) have been preserved to this day.

In 1896, the Paulinenstiftung became an independent deaconess motherhouse and in 1897 was elevated to the status of "Evangelische Kirchengemeinde Paulinenstift". In the following years, it took over facilities as outstations and branch institutions, such as the Elisabeth-Heilanstalt in Luisenstraße 45, Haus Goetz in Sonnenberger Straße 16-18, the deaconess home in Emser Straße 29 and the Von-Wintzingerode-Stift in Emser Straße 30.

By 1914, there were already 155 nurses working at the Paulinenstiftung. The hospital was expanded to 185 beds by 1915. Numerous nurses were trained in nursing here. In 1919, the Paulinenstiftung received state recognition as a nursing school for infants. The hospital served as a military hospital during both world wars and was confiscated by the US military administration in 1945. In 1947, the Paulinenstiftung merged with the sisterhood of the deaconess motherhouse from Łódz' in Poland, which had fled to the West and also included sisters from Sarata (Romania) and Warsaw.

In 1962-66, a new building with a 13-storey ward block was constructed on Schiersteiner Straße. In 1974, the foundation was renamed Diakoniegemeinschaft Paulinenstift. Since 1994, the hospital has been privately run under the name Asklepios Paulinen Klinik GmbH. Today, the Diakoniegemeinschaft Paulinenstift is a shareholder in the Gemeinschaftsklinikum Mittelrhein and also supports projects in care and education as a sponsoring foundation.